What does the FeedPlate® do?
The FeedPlate® allows the simulation of a Fed-Batch cultivation on a very small scale. Using the FeedPlate®, you can easily obtain reliable statements how your strains behave under Fed Batch conditions in the screening process.
What is the FeedPlate®?
The FeedPlate® consists of a commercially available microtiter plate with our special polymer matrix at the bottom of each well immobilized. This matrix contains the substrate.
How does the FeedPlate® work?
The FeedPlate® is a polymer-based release system. After the microtiter plate comes into contact with culture medium, the release of the substrate starts according to an evendefined rate over a period of up to 7 days.
Do I need special media to work with the FeedPlate®?
No. The Feed Plate® is a physicochemical system and does not require enzymes or special media or additives. The release kinetics is mainly dependent on the temperature and osmolarity of the medium used. Therefore, we refer to the release rate in the product catalog to a reference medium or cultivation conditions.
How many samples can I process with the FeedPlate®?
The FeedPlate® is currently available as standard in 24-well, 48-well and 96-well format, which allows you to process many samples in parallel in a reliable manner. This allows you to screen easily and quickly in high throughput.
What is the format of the FeedPlate®?
The FeedPlate® is available in commercially available microtiter plate formats. You choose which format you want to use. We produce special formats on request.
However, I only use microtiter plates from a certain manufacturer.
No problem, we can theoretically dose our matrix into any type of microtiter plate (cell culture tubes, 6-, 12-, 24-, 48- or 96-well MTPs), also different materials (PP, PS, etc.) or plates from certain manufacturers are possible (on request).
Are substrates other than C sources possible?
In addition to the standard carbon source glucose, we also offer other substrates such as maltose and fructose, and complex media such as yeast extract or other substrate classes such as nitrogen and phophate are also possible. Please contact us!
I need a high OTR (oxygen transfer rate). Can I still work in the 96 Deepwell plate?
Yes, that is not a problem. As long as you cultivate in Fed-Batch mode, oxygen supply should not be a problem. If you have any questions about how to reach a specific OTR, we will be happy to advise you and provide appropriate literature.
How should the FeedPlate® be stored?
The FeedPlate® should be stored dry at room temperature. Direct sunlight should be avoided.
How stable is the FeedPlate®?
The substrates are usually incorporated into the matrix as stable crystalline substances. Shelf life is therefore a question of sterility. The Feed Plate® is sterilized by beta radiation and is at least durable for one year.
You have a question that we do not answer here?
Contact us: Call us or write us an e-mail. We will be happy to answer your open questions.